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This is considered more realistic than pure in vitro testing because the natural architecture and cell-to-cell communication is maintained

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E., Forbes, G., & Halliday, D

The type III ANOVA analysis via Satterthwaites degrees of freedom was performed using the R package lmerTest [47] to assess the significance of the main effects, whereas significant relationships were further explored via estimated marginal means analysis using the package emmeans V1.11.1 [48], thus, a Tukey-adjusted contrasts pair wise comparison was also carried out

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